| 货号: 26904 |
| 规格: 30 µL |
| 基因符号: RHOA |
| 描述: Anti-RhoA-GTP Mouse Monoclonal Antibody |
| 背景介绍: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction. |
| 免疫原: Recombinant full length protein of active RhoA |
| 经过测试的应用: IP, IHC,IF |
| 推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG2b |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-RhoA monoclonal antibody recognizes active RhoA from vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western Blot: IP/WB Analysis of Active RhoA Protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-RhoA-GTP monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26007). |