Anti-Rheb-GTP Mouse Monoclonal Antibody

货号: 26910

规格: 30 µL

基因符号: RHEB

描述: Anti-Rheb-GTP Monoclonal Antibody

背景介绍: Small GTPases are a super-family of cellular signaling regulators. Rheb is a member of the Ras-superfamily GTPases. Rheb has been shown to interact with C-Raf, Mammalian target of rapamycin (mTOR), TSC2, Ataxia telangiectasia mutated (ATM), KIAA1303 and Ataxia telangiectasia and Rad3 related (ATR).

免疫原: Recombinant full-length protein of active Rheb.

经过测试的应用: IP, IHC,IF

推荐稀释比:
IP: 1 µg for 1~2 mg total cellular proteins
IHC, IF: 1:50-1:250

浓度: 1 mg/ml

宿主: Mouse

形式: Liquid

克隆性: Monoclonal

亚型: IgM

纯度: Purified from ascites

防腐剂: No

Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol

种属反应性: Anti-Rheb-GTP monoclonal antibody recognizes active Rheb of vertebrates.

储存条件: Store at -20°C. Avoid freeze/thaw cycles

免疫沉淀/蛋白质印迹:
Rheb activation assay. Purified Rheb proteins were immunoprecipitated after treated with GDP (lane 1) or GTPγS (lane 2). Immunoprecipitation was done with the anti-Rheb-GTP monoclonal antibody (Cat. No. 26910). Immunoblot was with an anti-Rheb rabbit polyclonal antibody (Cat. No. 21098).

Anti-Rheb-GTP Mouse Monoclonal Antibody

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