人抗鼠抗体(HAMA)ELISA试剂盒

人抗鼠抗体(HAMA)ELISA试剂盒产品基本参数：

【产品名称】：人抗鼠抗体(HAMA)ELISA试剂盒

【产品用途】：被测样品定性定量分析

【检测方法】：酶免法/酶联免疫法（ELISA）

【产品规格】：96T/48T（两种规格）

【运输条件】：低温运输，用干冰或者冰袋低温运输

【样品形式】：细胞培养上清/血清/血浆/其他生物液体（具体情况请咨询）

【独具特点】：灵敏和一致的性能；用血清血浆、组织培养上清或裂解产物进行过验证；半天内可完成的方便的检测。

Assay procedure

1.  Prepare all reagents before starting assay procedure. It is recommended that all Standards and Samples be added in duplicate to the Microelisa Stripplate.

2.  Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.

3.  Add Sample: Add testing sample 10μl then add Sample Diluent 40μl to testing sample well; Blank well doesn’t add anyting.

4.  Add 100μl of HRP-conjugate reagent to each well, cover with an adhesive strip and incubate for 60 minutes at 37°C.

5.  Aspirate each well and wash, repeating the process four times for a total of five washes. Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifold dispenser or autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating or decanting. Invert the plate and blot it against clean paper towels.

6.  Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.

7.  Add 50μl Stop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not

appear uniform, gently tap the plate to ensure thorough mixing.

8.  Read the Optical Density (O.D.) at 450 nm using a microtiter plate reader within 15 minutes.